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Exploring CIMac PrimaS™ for Purity Analysis of mRNA

CIMac PrimaS is a new member of BIA’s family of high performance chromatography products for analysis and purification of mRNA. Its positive charge gives it some anion exchange behavior but hydrogen bonding makes its selectivity is entirely distinct from traditional anion exchangers. QA and DEAE anion exchangers need to be heated into the range of 50–70°C for large mRNA to elute. Large mRNA elutes from CIMac PrimaS at ambient temperature in an ascending pH gradient. New experimental data show that large mRNA can also be eluted from CIMac PrimaS at ambient temperature in a pyrophosphate gradient. Resolution between in vitro transcription (IVT) contaminants and mRNA can be controlled with pH.